Journal: Cell Transplantation
Article Title: Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia
doi: 10.1177/09636897211041585
Figure Lengend Snippet: DOR activation with DADLE regulated p-synapsin I by BDNF-p-ERK pathway after global ischemia. Western blot analysis of BDNF (A), p-ERK1/2 (B), p-synapsin I (C) expression in rat hippocampus on day 3(left) and day 7(right) after global ischemia. ( N = 3 per group) * P < 0.05. ** P < 0.01. *** P < 0.001.
Article Snippet: After blocking with 5% skim milk, the membranes were incubated with primary antibodies against BDNF (1:100, Santa Cruz Biotechnology), p-ERK1/2, PKCα, β-actin, GAPDH (1:1000, Cell Signaling Technology), p-synapsin I, phospho-myristoylated alanine-rich C kinase substrate (p-MARCKS, 1:1000, Signalway Antibody), PKCε, PKCγ (1:1000, Proteintech Group) overnight at 4°.
Techniques: Activation Assay, Western Blot, Expressing